RHEM-AdV plateform
The Histology platform of the Arnaud de Villeneuve (AdV) campus is part of the Experimental Histology Network of Montpellier (RHEM), one of the platforms of Montpellier Biocampus Research Support Unit. Its mission is to assist users in histological processing of tissues from animal or human models, with a particular specialization in the RNAscope™ approach. This platform provides access to equipments and offers technical and scientific support to academic research teams and private companies expressing the need for it.
Additionally, the RHEM-AdV platform offers expertise in non-toxic clarification (X-Clarity) as well as in situ hybridization using the RNAscope technology. If you wish to become a user of the platform for equipment provision or service requests, please contact the platform at the generic address RHEM-AdV@rhem.cnrs.fr.
RHEM-AdV is a BioCampus platform.
RNAscope in situ hybridization showing the expression pattern of the genes Vip (yellow) and Period1 (red) in the suprachiasmatic nuclei of the mouse hypothalamus. Cell nuclei are stained with DAPI (blue).
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2016Accreditation to supervise research (HDR) - Neurosciences - University Montpellier - France
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1999PhD in Neurosciences - University Montpellier - France
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1994MSc in Pharmacology - University Montpellier - France
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1994Engineer Diploma in Chemistry & Biochemistry - Chemistry Engineering School - Montpellier - France
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2009-CNRS Faculty Research Scientist - Institute of Functional Genomics (IGF) - Montpellier - France
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2002-2008CNRS Faculty Research Scientist, Dr A. Privat Team - Institute for Neurosciences (INM) - Montpellier - France
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1999-2002Postdoctoral fellow (Dir. Pr J.M. Henley) - Department of Anatomy, MRC Centre for Synaptic Plasticity - University of Bristol - England
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1999Assistant Professor (Dir. J. Vignon) - INSERM U336, Chemistry School - Montpellier - France
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1994-1999PhD student (Dir. J. Vignon) - INSERM U336, Chemistry School - Montpellier - France
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2023-Head of GDR "Microglia and Neuroinflammation"
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2021-Scientific Manager of the RHEM-AdV histology platform (i.e., Montpellier Histology Platform, Arnaud de Villeneuve campus)
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2019-Member of the GDR "Microglia and Neuroinflammation" steering committee
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2019-Member of the Glial Cell Club Board, Treasurer
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2019-Review Editor for Frontiers in Aging Neurosciences
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2014-Member of the iExplore animal facility steering committee, Arnaud de Villeneuve site; project follow-up & Indexing of murine lines on the animal facility network; bi-monthly meetings
- My overall research interests are to study the pathophysiological mechanisms that lead to neurodegenerative diseases (ND).
- My current researches aim at deciphering the contribution of glial cells, in particular microglial cells, to the initiation and progression of these pathologies, in particular Alzheimer's Disease. More specifically, I aim to characterize the early microglial response and identify its role in ND pathogenesis.
- The ultimate goals of my researches are to identify the microglial molecular signaling pathways involved, and to determine whether these can help identify disease biomarkers or represent targets for the development of innovative therapeutic strategies. We are currently focusing on Clec7a/Dectin-1 receptor, a pattern recognition receptor identified as an important regulator of peripheral inflammation but whose microglial role remains largely unknown.
- To achieve our objectives, we use a wide range of complementary technological approaches, including :
- glial cell purification
- transcriptomic analysis (qPCR, dPC, Nanostring, RNAseq & scRNAseq) on isolated cells
- primary cultures of microglia and hiPSCs (human induced pluripotent stem-cells)
- cellular approaches (phagocytosis, cytokine release)
- histological analyses (IHC, RNAscope)
- behavioral analysis (Barnes-Maze, Open-field; Y-Maze)
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Publications external to IGF
- Fluorescent non transgenic schistosoma to decipher host-parasite phenotype compatibility. D. Duval, P.Poteaux, B. Gourbal, A. Rognon, R. De Carvalho Augusto (2023) Front. Immunol. 27:14:1293009. doi: 10.3389/fimmu.2023.1293009.
