Team Stéphanie TROUCHE
Appetitive and aversive memory circuits
Our lives are built around positive and negative experiences embedded in particular places, contexts and states. These rewarding or punishing experiences can recruit distinct, but also overlapping, neuronal ensembles in different brain circuits, underlying appetitive and aversive memories. The overall goal of our team is to understand how appetitive and aversive neuronal circuits are shaped by past experience, environment (spatial or social) and internal state, and how they interact to select appropriate behaviors such as approach or avoidance.
To reach this goal, we employ in vivo and ex vivo recordings, intersectional viral strategies, pharmacogenetics, close-loop optogenetic intervention, automated behavioural tracking and in situ hybridization in mice. By visualizing, monitoring and manipulating precise neuronal ensembles, the team aims to provide a mechanistic understanding of the cross-talk between the reward and aversive systems. This knowledge will advance our theoretical understanding on psychiatric disorders for which emotional and decision-making processes are altered (e.g., PTSD).
Funding
IGF Nord 113
04 34 35 92 76
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2020Habilitation to Supervise Research (HDR) - University of Montpellier - France
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2009PhD in Neuroscience - University Paul Sabatier - Toulouse - France
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2023-New group leader "Appetitive and aversive memory circuits" team - Institute of Functional Genomics - Montpellier - France
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2018-Inserm Researcher - Institute of Functional Genomics - Montpellier - France
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2012-2018MRC Investigator Scientist - University of Oxford - Oxford - UK
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2010-2012Postdoctoral Research Fellow - Tufts University - Boston - USA
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2022Nominated member of the Fyssen Foundation lecture committee
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2017"Young Investigator Grant” NARSAD, BBRF, USA
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2014-2016Special Award in recognition of research performances, Medical Research Council, Brain Network Dynamics Unit, Oxford, UK
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2010Postdoctoral Outgoing Fellowship from the Fyssen Foundation
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2010Young Researcher Prize from the Bettencourt Schueller foundation
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2010Research Fellowship from the Philippe Foundation France-USA (2 years)
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2010Thesis prize from “Le Monde”
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2010Thesis prize from the University Paul Sabatier
- The general goal of my research is to comprehensively study the interaction of the appetitive and aversive memory systems driving appropriate behaviour in mice. While most work studied the neural circuits of appetitive or aversive memories separately, our team challenges this dichotomic view by examining the appetitive-aversive memory circuit interaction from the behavioural to the neuronal and molecular angle.
- To pinpoint the precise nature of dysfunction in these memory systems, we aim to dissect the underlying neural circuits and manipulate them with a high spatio-temporal resolution to specifically correct impaired behaviour.
- To this effect, I have employed throughout my career in neuroethology and neurophysiology, multi-faceted anatomical and electrophysiological approaches in behaving transgenic mice, combining cell-type viral strategies with in vivo multi-channel recordings, fiberphotometry, optogenetics, pharmacogenetics and functional anatomy techniques.
IGF Nord 113 04 34 35 92 76
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2007PhD - Neurobiology - University of Montpellier II - France
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2003MSc - Neurobiology of Communication and Integration Processes - University of Montpellier II - France
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2000BSc - Cellular Biology and Physiology - University of Montpellier II - France
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2019-CNRS Researcher (CRCN) - Institute of Functional Genomics- Montpellier - France
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2015-2018CNRS Researcher (CR2) - Institute of Functional Genomics- Montpellier - France
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2012-2014Principal investigator - Brain Research Institute - Zürich - Switzerland
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2007-2012Postdoctoral fellow - Brain Research Institute - Zürich - Switzerland
- Distinctions
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2013Swiss foundation for Excellence and Talent in Biochemical Research
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2006PhD fellowship from the Fondation pour la Recherche Médicale (FRM)
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2003Ministerial PhD fellowship (MRT)
- Responsabilities
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2022-Member of the Jean Ster Foundation
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2022-Member of the IT commission - Institute of Functional Genomics
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2021-Member of the CoPil of the Genobi – Montpellier genotyping platform
- I am an electrophysiologist based on my neuroscience background, specializing in in vitro recordings on acute / organotypic slices of mouse brain. My research interests is to understand the cellular and molecular mechanisms involved in the regulation of synaptic strength at excitatory and inhibitory synapses (synaptic plasticity). As well as studying how synaptic transmission gives rise to complex behaviors.
- Electrophysiological techniques
- Patch-clamp recording in whole-cell configuration (classical and patch-perforated)
- Double-recording of synaptically connected cells
- Recordings of unitary channels
- Extracellular field recording (fEPSP)
- Cultures
- Cell line cultures
- Primary neuronal cultures
- Organotypic slices
- In-situ Hybridation (RNAscope)
- Cellular biology & biochemistry (Western blot, Co-immunoprecipitation, Biotinylation Assay, Endocytosis Experiment)
- Immunohistochemistry
- Designer Level 1 and surgery trainings
- Data analysis
- Clampex/Clampfit
- GraphPad Prism
- Adobe Illustrator
- Image J
IGF Sud 113 04 34 35 92 76
IGF Nord 113 04 34 35 92 76
- Graduate studies
- - 14 March 2019 (1 April 2014-14 March 2019). PhD at the Faculty of Natural and Exact
- Sciences, University of Buenos Aires, Argentina. Topic: “Neurobiological mechanisms involved
- in pattern separation of long-term memory storage”. Advisor: Dr.Pedro Bekinschtein
- Undergraduate studies
- -10 March 2014 (20 March 2008-10 March 2014). Bachelor's Degree in Biological Sciences,
- School of Natural and Exact Sciences, University of Buenos Aires, Argentina. Major: Physiology
- and Molecular Biology.6 years professional degree with thesis “Environmental enrichement as a
- therapeutical strategy for retinal ischemic damage”. Advisor: Dr.Ruth E. Rosenstein. Average
- Career Grade: 8.74
- -1st July 2022 to 31 June 2025.
- Postdoctoral Research Fellowship granted by ‘Human Frontiers Science Program
- Organization’ at the Laboratory of Appetitive and Aversive Memory Circuits lab of the
- Institute of Functional Genomics. Advisor: Dr. Stephanie Trouche. Topic. Neural basis of
- relative aversive value coding in mice.
- -1st April 2021 to 31 June 2022.
- Postdoctoral Research Fellowship granted by ‘Foundation Fyssen’ at the Laboratory of
- Appetitive and Aversive Memory Circuits lab of the Institute of Functional Genomics.
- Advisor: Dr. Stephanie Trouche. Topic. Role of the Paraventricular Thalamus in relative
- aversive value based learning and choice in mice.
- -1st April 2019 to 1st April 2021
- Postdoctoral Research Fellowship granted by CONICET at the Laboratory of Memory
- Research and Molecular Cognition lab of the Institute of Cognitive and Translational
- Neuroscience. Advisors: Dr. Pedro Bekinschtein and Dr. Agustín Ibañez. Topic. Changes in
- memory discrimination and generalization abilities in humans during normal aging.
- -1st April 2014 to 1st April 2019
- Graduate Research Fellowship granted by CONICET to complete the PhD at the
- Laboratory of Memory Research and Molecular Cognition lab of the Institute of CellBiology and Neuroscience of the School of Medicine, University of Buenos Aires,
- Argentina. Advisor: Dr. Pedro Bekinschtein.
- -July 2011 to March 2014
- Internship at the Retinal Neurochemistry lab. Department of Biochemistry, School of
- Medicine, University of Buenos Aires, Buenos Aires, Argentina. Advisor: Dra. Ruth E.
- Rosenstein.
- -Guest Editor of Special Issue ‘Recent Advances in the Stabilization and Integration of Associative Memories.’, Frontiers in Behavioral Neuroscience 2024.
- -Guest Editor of Special Issue ‘Updates on memory modulation in health and disease.’ Frontiers in Behavioral Neuroscience 2023.
- -Human Frontiers Postdoctoral Fellowship 2022.
- -Fyssen Postdoctoral Fellowship 2020.
- -Postdoctoral grant CONICET 2019.
- -PhD grant CONICET 2014.
- - Travel award IBRO to attend the Neurodynamics Symposium, Grenoble, France, 2019 July 4-5.
- -Travel award for attending the “Young Investigator Training Program FENS Forum 2018”.
IGF Nord 113 04 34 35 92 76
IGF Nord 113 04 34 35 92 76
- Perisse E, Miranda M and Trouche S. Modulation of aversive value coding in the vertebrate and invertebrate brain. Current Opinion in Neurobiology, 2023 Ap:79:102696.
- Tuduri P*, Bouquier N*, Girard B*, Moutin E, Thouaye M, Perroy J, Bertaso F, Ster J. Modulation of hippocampal network oscillation by PICK1-dependent cell surface expression of mGlu3 receptors. Journal of Neuroscience, 2022. * contribution égale.
- Sasaki Russell J, Trouche S and Reijmers LG. Functional characterization of the basal amygdala – dorsal BNST pathway during contextual fear conditioning. eNeuro, 2020 Jul 13;7(4):.0163-20.2020.
- Trouche S, Pompili N and Girardeau G. The role of sleep in emotional processing: insights and unknowns from rodent research. Current Opinion in Physiology, 2020 Jun; p230-237.
- Trouche S, Koren V, Doig NM, Ellender TJ, El-Gaby M, Lopes-dos-Santos V, Reeve HM, Perestenko PV, Garas FN, Magill PJ, Sharott A and Dupret D. A hippocampus-accumbens tripartite neuronal motif guides appetitive memory in space. Cell, 2019 Mar 7;176(6):1393-1406.e16.
- Berry S, Weinmann O, Fritz AK, Rust R, Wolfer D, Schwab ME, Gerber U, Ster J. Loss of Nogo-A, encoded by the schizophrenia risk gene Rtn4, reduces mGlu3 expression and causes hyperexcitability in hippocampal CA3 circuits. PLoS One, 2018; 3(7):e0200896.
- Rosenberg N, Gerber U and Ster J. Activation of Group II Metabotropic Glutamate Receptors Promotes LTP Induction at Schaffer Collateral-CA1 Pyramidal Cell Synapses by Priming NMDA Receptors. Journal of Neuroscience, 2016; 36(45):11521-11531.
- Trouche S, Perestenko PV, van de Ven GM, Bratley CB, McNamara CG, Campo-Urriza N, Black SL, Reijmers LG, Dupret D. Recoding a cocaine-place memory engram to a neutral engram in the hippocampus. Nature Neuroscience, 2016 Apr;19(4):564-7.
- McNamara CG, Tejero-Cantero A, Trouche S, Campo-Urriza N, Dupret D. Dopaminergic neurons promote hippocampal reactivation and spatial memory persistence. Nature Neuroscience, 2014 Dec 17(12):1658-60.
- Trouche S, Sasaki J, Tu T, Reijmers LG. Fear extinction causes target specific remodeling of basal amygdala perisomatic synapses. Neuron, 2013 Nov20;80(4):1054-65.
Interaction between appetitive and aversive memory circuits
Principal investigator
Stéphanie TROUCHE
2024
• E. Debos (Master 2 Neuroscience, 6 mois).
• C. Sanié, O.Hamidi (Master 1 Neuroscience, 6 mois).
• Tebar (IUT Génie Biologique, 6 semaines).
2023
• C. Bernard (Master 1 MER, 2 mois).
• E. Larche (Master 1 Neurosciences, 6 mois).
• S. Thomas, J.Mameri (L3 APP, 6 semaines)
• E. Baert, M.Bierry, L.Baltimore (L3, 6 demi-journées).
2022
• E. Karam (Master 2 MER, 6 mois),
• L. Izquierdo (L1, 6 semaines).
2021
• P. Fourquin, Vanessa Greggio, Lea Beaujoin (L3, 6 demi-journées).
• L. Vaillant (EPHE Master 2, 6 mois).
2020
• J. Beauduffe (Master 2 Neuroscience, 5 mois).
• J. Grelaud (Master 1 Neuroscience, 6 mois).
• J. Tram (Doctorante-mentee de l’association « Femmes et Sciences », 6 mois).
2019
• E. Abi Habib (doctorante-mentee de l’association « Femmes et Sciences », 6 mois).
• J. Bertin (Master 1 Neuroscience, 4 mois et demi).
• M. Verdy (Master 1 MER, 6 mois).
Public outreach
2024
• Brain week, Mermoz high school, « Cerebral Plasticity», Montpellier, France (3 days, Magdalena Miranda).
2023
• Zoom session Neuromentoring, “How to find a lecturer/researcher position in France?” (Elsa Karam).
• Declic Days, Georges Pompidou high school, Castelnau-le-lez, France (Majda Drahmani).
2022
• Declic Days, Georges Pompidou high school, Castelnau-le-lez, France (Elsa Karam and Stephanie Trouche).
• Declic Days, Georges Pompidou high school, Castelnau-le-lez, France (Majda Drahmani).
• Science festival booth “Super Power of bacteria”, Montpellier, France (Elsa Karam).
Science festival, Village des Sciences, Montpellier (workshop with 40 primary school children, Stéphanie Trouche).
2019
• Conference “Quai des Savoirs”, Toulouse, France.
• Conference at the University of Third Time « Spatial memory and navigation » and «Strategies for searching the memory trace » (Stéphanie Trouche).
2018
• Member of Women and Science association, Montpellier, France. Mentor for PhD students in Science (Stéphanie Trouche).
• Conference at the University of Third Time « Spatial memory and navigation » and «Strategies for searching the memory trace » (Stéphanie Trouche).
• Brain week, Jean Monnet high school, Montpellier, France (2 days, Jeanne Ster).
2017
• Brain week, Jean Monnet high school, Montpellier, France (2 days, Jeanne Ster).
