PPM-FPP plateform

Functional Proteomics Platform

Séverine Chaumont-Dubel
MCF, UM
Martial Seveno
IR1, CNRS

The Proteome Pole of Montpellier (PPM) is one of the life science core facilities of the service unit BioCampus Montpellier. The PPM federates 4 sites of expertise in proteomic analysis, including the Functional Proteomics facility (FPP) located at the Institute of Functional Genomics.
The PPM is one of the 6 nodes of the ProFi national proteomics infrastructure. It has held the IBiSA label since 2009 and ISO 9001:2015 certification since 2015.

The Functional Proteomics facility (FPP) offers dual expertise and cutting-edge technologies in:
• High-resolution mass spectrometry for protein identification and quantification, differential analysis (with or without labeling) and protein partner discovery.
• Structural proteomics for the study of protein-protein and protein-ligand interactions, as well as their structural dynamics under native conditions.

Expertise and services
• Preparation of biological samples (purification, separation, enrichment and prefractionation) for mass spectrometry analysis,
• Targeted and large-scale quantitative proteomics,
• Structural analysis by mass spectrometry,
• Study of post-translational modifications,
• Mass spectrometry-based interactomics studies,
• Mass spectrometry-based biomarker discovery and validation,
• Bioinformatics and statistical analysis of the data produced.

Contact
For all requests for services or scientific collaboration, please contact the core facility with a brief description of your requirements.
A meeting with platform staff will be proposed as soon as possible to discuss the feasibility of your project, the strategies and techniques to be used, and the nature of the services the platform can offer you.

During this discussion, the financing and the type of project (service or collaboration) will also be defined according to the terms of the PPM charter. The time required to complete projects varies according to the type of service requested.

NanoSpray Ion Source (Thermo Fisher Scientific)

Researchers
Cherine
Bechara
PU, UM
  IGF Sud 025
  04 34 35 93 24
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Séverine
Chaumont-Dubel
MCF, UM
  IGF Sud 029
  04 34 35 92 17
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Technicians and engineers
Mathilde
Decourcelle
IECN, CNRS
  IGF Sud 029
  04 34 35 92 17
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Khadija
El Koulali
AI, CNRS
  IGF Sud 029
  04 34 35 92 17
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Martial
Seveno
IR1, CNRS
  IGF Sud 029
  04 34 35 92 17
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Serge
Urbach
IR1, CNRS
  IGF Sud 029
  04 34 35 92 17
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Oana
Vigy
IEHC, CNRS
  IGF Sud 029
  04 34 35 92 17
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Fixed term contract technicians and engineers
Tristan
Girbau
IE CDD, Inserm
  IGF Nord 207c
  04 34 35 92 64
Know more >

Major Publications
  • Picard MAL, Leblay F, Cassan C, Willemsen A, Daron J, Bauffe F, Decourcelle M, Demange A, Bravo IG. Transcriptomic, proteomic and functional consequences of codon usage bias in human cells during heterologous gene expression. Protein Sci. 2023 Mar;32(3):e4576. doi: 10.1002/pro.4576. PMID: 36692287
  • Rebendenne A, Roy P, Bonaventure B, Chaves Valadão AL, Desmarets L, Arnaud-Arnould M, Rouillé Y, Tauziet M, Giovannini D, Touhami J, Lee Y, DeWeirdt P, Hegde M, Urbach S, Koulali KE, de Gracia FG, McKellar J, Dubuisson J, Wencker M, Belouzard S, Moncorgé O, Doench JG, Goujon C. Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs. Nat Genet. 2022 Aug;54(8):1090-1102. doi: 10.1038/s41588-022-01110-2. Epub 2022 Jul 25. PMID: 35879413
  • Lambey P, Otun O, Cong X, Hoh F, Brunel L, Verdié P, Grison CM, Peysson F, Jeannot S, Durroux T, Bechara C, Granier S, Leyrat C. Structural insights into recognition of chemokine receptors by Staphylococcus aureus leukotoxins. 2022 Mar 21;11:e72555. doi: 10.7554/eLife.72555. PMID: 35311641
  • Al Awabdh S, Donneger F, Goutierre M, Séveno M, Vigy O, Weinzettl P, Russeau M, Moutkine I, Lévi S, Marin P, Poncer JC. Gephyrin interacts with the K-Cl co-transporter KCC2 to regulate its surface expression and function in cortical neurons. J Neurosci. 2022 Jan 12;42(2):166-182. doi: 10.1523/JNEUROSCI.2926-20.2021. Epub 2021 Nov 22. PMID: 34810232
  • Fumagalli A, Heuninck J, Pizzoccaro A, Moutin E, Koenen J, Séveno M, Durroux T, Junier MP, Schlecht-Louf G, Bachelerie F, Schütz D, Stumm R, Smit MJ, Guérineau NC, Chaumont-Dubel S, Marin P. The atypical chemokine receptor 3 interacts with Connexin 43 inhibiting astrocytic gap junctional intercellular communication. Nat Commun. 2020 Sep 25;11(1):4855. doi: 10.1038/s41467-020-18634-y. PMID: 32978390

Find all PPM publications on the website