The Team focuses on the neuro-vascular unit (NVU) and its pharmacology. The Team investigates the pathophysiological role of cerebral vessels in contribuiting to neuronal dysfunction. The cerebrovascular tree has rheological, immunological and transport-metabolic functions, strictly controlling neuronal activity. Cerebral vessels include endothelial cells enclosed by a perivascular basal lamina, astrocytic end-feet and mural cells.
Neuro-vascular techniques used: i) in vivo video-electroencephalography and analysis of frequency signals; ii) 3D / 2D in vivo and in vitro imaging (2-photon or confocal). Fluorescent 3D brain reconstruction; iv) ex vivo electrophysiology (e.g., field potential); v) molecular biology (e.g., microvessel isolation, immunohistochemistry, western blot, RT-PCR). Models used include: i) in vivo models of seizures or Alzheimer's Disease; ii) ex-vivo organotypic culture; iii) in vivo and ex-vivo models of neuro-vascular dysplasia; iv) brain specimens from drug resistant epileptic or Alzheimer's patients.
Collaborative effort within the IGF: i) IPAM platform and Jeanneteau Team (in vivo 2-Photon imaging and reconstruction); ii) S. Clayesen (experimental models of AD and behavioral testing); iii) Rassendren Team (model of seizures and inflammation); iv) JM. Pascussi (PXR/CAR biology).
External collaborations: C. Besta Neurologic Hospital (Milan, Italy); Hopital La Timone (Marseille, France); INRA Toulouse (France); Cleveland Clinic (USA).