MOLECULAR MECHANISMS OF REGENERATION
Department: Physiology - Research axis : Biology of ion channels

Research subject

Heart disease has now become one of the most common causes of fatality. Indeed, many European countries report that heart disease is the number one cause of death and disability. Although adult mammals are unable to significantly regenerate their heart, this is not the case for a number of other vertebrate species. In particular, zebrafish are able to fully regenerate their heart following amputation of up to 20% of the ventricle.
Previously we developed the Cre/tamoxifen system for use in zebrafish and were able to lineage trace cardiomyocytes during regeneration. We found that soon after amputation, cardiomyocytes dedifferentiate and proliferate to regenerate the missing tissue, furthermore this process did not involve stem/progenitor cells. More recently, identical results have also been obtained in neonatal mice, indicating that mammals possess the potential to regenerate their heart in a similar manner to zebrafish. Our main objective is to identify genes which regulate myocardial regeneration in zebrafish and subsequently test these in mammals.

To determine suitable target genes, we will use the substantial microarray data generated from studying heart regeneration in zebrafish. To further refine the selection of candidates from the microarray data, we will target genes that are associated with specific processes that we have identified as being important for zebrafish heart regeneration. Once candidates have been selected and confirmed in our zebrafish model system we will begin testing these in vitro in mammalian cardiomyocytes before creating transgenic mouse models to determine whether they are capable of inducing myocardial regeneration following cardiac ischemia in mammals

 

 

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Team

Team leader

Chris Jopling
CR1, Inserm


  IGF Sud 130

  04 34 35 92 53

 

Staff

Anaïs Bertaud
Licence 3, CNRS


  IGF Sud 130

  04 34 35 92 53

 

Adèle Faucherre
CR1, CNRS


  IGF Sud 130

  04 34 35 92 53

 

Hamid Moha
MCF, UM


  IGF Sud 130

  04 34 35 92 53

 

Nathalie Nasr
Doctorant(e), CNRS


  IGF Sud 130

  04 34 35 92 53

 

Pierre Rambeau
Doctorant(e), CNRS


  IGF Sud 130

  04 34 35 92 53

 

Monia Souidi
Master 2, UM


  IGF Sud 130

  04 34 35 92 53

 

Leïla Talssi
Master 2, UM


  IGF Sud 130

  04 34 35 92 53

 


Major publications

  • Faucherre A, Nargeot J, Mangoni ME, Jopling C. piezo2b Regulates Vertebrate Light Touch Response.J Neurosci. 2013 Oct 23;33(43):17089-94. doi: 10.1523/JNEUROSCI.0522-13.2013. PMID: 24155313 [PubMed - in process]
  • Faucherre A, Kissa K, Nargeot J, Mangoni M, Jopling C. Piezo1 plays a role in erythrocyte volume homeostasis.Haematologica. 2013 Jul 19. [Epub ahead of print]
  • Jopling C, Suñe G, Faucherre A, Fabregat C, Belmonte-Izpisua JC. Hypoxia induces myocardial regeneration in zebrafish.Circulation. 2012 Nov 14.
  • Faucherre A and Jopling C. The heart’s content-renewable resources.Int J Cardiol. 2012 Oct 5 S0167-5273(12)01157-6. Review.
  • Jopling C, Sune G, Morera C, Belmonte JC. p38a MAPK regulates myocardial regeneration in zebrafish.Cell Cycle. 2012 Mar 11, 1-7.
 

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