DEVELOPMENT OF CEREBELLAR GABAERGIC CIRCUIT
Department : Neuroscience - Research axis : Molecular & cellular networks

 

Research subject

Perception and behavior are critically dependent on synaptic communication between specific neurons. Understanding how neurons achieve such “synaptic specificity” is therefore one of the most fundamental issues in developmental neuroscience.

In the laboratory, we are studying the development of GABAergic cerebellar circuit. GABAergic inhibitory circuits consist of an array of interneuron cell types with distinct morphology, physiological properties, and synaptic connectivity patterns, and may constitute a scaffold to organize local cortical connectivity and activity. Indeed, one of the most puzzling feature of GABAergic interneurons is their specific subcellular domains innervation (i.e; soma, spines, dendrite and axon hillock) of their target neurons which allowed strong and precise control of synaptic input, integration and output patterns. Although GABAergic connectivity is essential for the construction of functional circuits, the formation and development of exquisite GABAergic circuits, including migration, axon guidance and synaptogenesis, in general are poorly characterized.

By using several mouse genetic models, the laboratory established extensive experimental approaches in model systems to study specific GABAergic function both in vitro and in vivo. The principal objective of the laboratory is to characterize the early event of specific GABAergic development, and to identify cellular and molecular mechanisms of several key stages of GABAergic interneurons migration, axon guidance and synaptogenesis.

 

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Team

Team leader

Fabrice Ango
CRCN, CNRS


  IGF Sud 225

  04 34 35 92 01

 

Staff

Raphael Dos-Reis
Doctorant(e), UM


  IGF Sud 225

  04 34 35 92 01

 

Céline Jahannault-Talignani
TCN, UM


  IGF Sud 225

  04 34 35 92 01

 


Major publications

  • Telley L, Cadilhac C, Cioni JM, Huber AB, Faivre-Sarrailh C, Jahannault-Talignani C, Dayer A, Huber AB and Ango F. Dual function of NRP1 in axon guidance and subcellular target recognition in cerebellum. 2016 Neuron. 2016 Sep 21;91(6):1276-91.
  • Saywell V, Cioni JM, Ango F. Developmental gene expression profile of axon guidance cues in Purkinje cells during cerebellar circuit formation. 2014. Cerebellum 13(3):307-17.
    Cioni JM, Telley L, Saywell V, Cadilhac C, Jourdan C, Huber AB, Huang JZ, Jahannault-Talignani C, Ango F. SEMA3A signaling controls layer-specific interneuron branching in the cerebellum. 2013. Curr Biol. 23(10):850-61.
  • Ango F, Wu C, Van der Want JJ, Wu P, Schachner M, and Huang ZJ. ( Bergmann glia and the recognition molecule CHL1 organize GABAergic axons and direct innervation of Purkinje cell dendrites. PLoS Biol. 2008 Apr 29;6(4):e103. doi: 10.1371/journal.pbio.0060103.
  • Huang ZJ, Di Cristo G, Ango F.Development of GABA innervation in the cerebral and cerebellar cortices. Nat Rev Neurosci. 2007 Sep;8(9):673-86
  • Ango, F., Di Cristo, G., Hagashiyama, H., Bennett, V., Wu, P. and Z. Josh Huang. Ankyrin-based subcellular gradient of the immunoglobulin family protein neurofascin specifies synaptic innervation at axon initial segment of Purkinje neurons. Cell. 2004 Oct 15;119(2):257-72.

 

 

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