Research

The Granier Lab aims at characterizing the molecular and structural mechanisms of GPCRs and PAQRs function. Both of these receptor families present a 7TM which acts as a receptor and a transducer of extracellular signals within the cell to regulate a wide variety of physiological function.

 

As such, they are the subject of intense studies both in academic labs and in industry with important drug discovery programs. Despite recents advance in the field, there is still an important lack of knowledge of 7TM signalling mechanisms and pharmacology. This is mainly due to the challenges associated with the characterisation of membrane protein structure and function.

To fill this gap of knowledge, we are developing an innovative integrated strategy combining state-of-the-art molecular and structural pharmacology approaches and computational methods including :
 
 

Approach I

Molecular Pharmacology

Molecular analyses of transmembrane signalling network using resonance energy transfer measurement in living cells and proteomic analyses
 

Approach II

Structural Biology

Structural analyses of receptors and signalling complexes using biochemistry, fluorescence, NMR spectroscopy and X-ray crystallography.
 

Approach III

Molecular Dynamics

Molecular dynamics simulations reveal the dynamics and mechanisms of proteins at atomic-level .

 

BRET/FRET cells based

NMR spectroscopy

Modelisation & SAXS

 

X-ray crystallography

Cryo-electron microscopy