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Membrane receptors : structure, dynamics and pathology
Technicals skills
par - 20 mai 2012
Molecular biology : cDNA cloning, PCR, RT-PCR, site-directed mutagenesis, sequencing, vectors
Culture of cells : transfection, eukaryotic and bacterial cell lines
Pharmacology : ligand binding, second messengers measurement, design and synthesis of photoactivatable, fluorescent and radiolabeled ligands
Biochemistry : production and purification of receptors, photoaffinity labeling, immunostaining, mass spectrometry, circular dichroism, fluorescence techniques
Cellular biology : proteomics, fluorescence microscopy.