Institut de
Génomique
Fonctionnelle

ANIMAL MODELS

• • 5-HT4 receptor knock-out (Compan et al. 2004).
  • 5-HT1B receptor knock-out (Saudou et al., 1994 : coll. R. HEN)
  • Tryptophane hydroxylase 2 knock-out (Gutknecht et al., 2012)

Underway : 5-HT4 Receptors Activated Solely by Synthetic Ligand 5-HT4 – RASSL mouse

EXPERIMENTAL DEVICES

• • Running wheels (Activity-based anorexia) and feeding or liquid preference
  • Metabolic cages
  • Self-administration (food, drugs)
  • Conditioned place preference
  • Open – field
  • Elevated plus maze

TECHNICS

• • Behavioral tests (cf. supra)
  • Intracerebral microsurgery combined to pharmacological treatment in behaving animals
  • Molecular biology Cloning, site-directed mutagenesis, RQ-PCR, In situ hybridization, siRNA and gene transfer in vivo (viral and JetPEI vectors)
  • Biochemistry HTRF cAMP in cerebral and peripheral tissues, autoradiography, western – blot, radioimmunoassay
  • Immunocytochemistry
  • Knockout strategy